bioI [2019-08-09 07:37:21]

cytochrome P450 enzyme

Locus: BSU_30190

Isoelectric point: 6.20

Molecular weight: 44.70 kDa

Protein length: 395 aa

Gene length: 1188 bp

Function: biosynthesis of biotin

Product: cytochrome P450 enzyme

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of biotin

Gene

Coordinates: 3,089,226 3,090,413

The protein

Catalyzed reaction/ biological activity

• formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids

Protein family

• cytochrome P450 family (according to UniProt)

Paralogous protein(s)

• CypA, YjiB, PksS

Structure

• 3EJB, 3EJE (complex with AcpA) PubMed

Expression and Regulation

Operons

• Genes: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
  Description: PubMed

  Regulatory mechanism

  • BirA: repression, PubMed, in BirA regulon

  Regulation

  • repressed in the presence of biotin (BirA) PubMed

Biological materials

Mutant

• MGNA-A804 (bioI::erm), available at the NBRP B. subtilis, Japan
• BKE30190 (bioI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG
• BKK30190 (bioI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG

References

Reviews

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Original publications

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